Construct: shRNA TRCN0000106044

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013862.3-1971s1c1
DNA Barcode:
GAGTGTTATTACTCGAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rabgap1l (29809)
Gene Description:
RAB GTPase activating protein 1-like
Transcript:
RefSeq NM_013862.3 (NON-CURRENT)
Match location:
Position 1971 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106044)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGTTATTACTCGAGATAT
Hairpin Sequence:
5'-CCGG-GAGTGTTATTACTCGAGATAT-CTCGAG-ATATCTCGAGTAATAACACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGTTATTACTCGAGATATCTCGAGATATCTCGAGTAATAACACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGTTATTACTCGAGATATCTCGAGATATCTCGAGTAATAACACTC-3'

Other clones with same target sequence:

  1. TRCN0000315587