Construct: shRNA TRCN0000106303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030744.2-614s1c1
DNA Barcode:
CGTTAGCAGGATGTTAAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ropn1 (76378)
Gene Description:
ropporin, rhophilin associated protein 1
Transcript:
RefSeq NM_030744.2 (CURRENT)
Match location:
Position 614 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTAGCAGGATGTTAAACTA
Hairpin Sequence:
5'-CCGG-CGTTAGCAGGATGTTAAACTA-CTCGAG-TAGTTTAACATCCTGCTAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTAGCAGGATGTTAAACTACTCGAGTAGTTTAACATCCTGCTAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTAGCAGGATGTTAAACTACTCGAGTAGTTTAACATCCTGCTAACG-3'

Other clones with same target sequence:

(none)