Transcript: Mouse NM_030744.2

Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Ropn1 (76378)
Length:
1030
CDS:
78..716

Additional Resources:

NCBI RefSeq record:
NM_030744.2
NBCI Gene record:
Ropn1 (76378)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_030744.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106301 CCACGTTAGCAGGATGTTAAA pLKO.1 611 CDS 100% 13.200 10.560 N Ropn1 n/a
2 TRCN0000106302 GCAGTTTACCAAAGATGCCAT pLKO.1 134 CDS 100% 2.640 2.112 N Ropn1 n/a
3 TRCN0000106303 CGTTAGCAGGATGTTAAACTA pLKO.1 614 CDS 100% 5.625 3.938 N Ropn1 n/a
4 TRCN0000140466 GAGATCGAGTGGCTGAAGTTT pLKO.1 423 CDS 100% 5.625 3.938 N ROPN1B n/a
5 TRCN0000106300 CCATGCACCATCTAAAGTCAA pLKO.1 781 3UTR 100% 4.950 3.465 N Ropn1 n/a
6 TRCN0000106304 GATGGCTTAATCAAGGTGAAT pLKO.1 660 CDS 100% 4.950 3.465 N Ropn1 n/a
7 TRCN0000141691 CAGGAAGTAATTGGTCCTGAT pLKO.1 642 CDS 100% 0.405 0.243 N ROPN1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030744.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03456 pDONR223 100% 85.5% 83.4% None (many diffs) n/a
2 ccsbBroad304_03456 pLX_304 0% 85.5% 83.4% V5 (many diffs) n/a
3 TRCN0000469769 TCGGCTGCCCCAACCTTCTTTAAG pLX_317 64.7% 85.5% 83.4% V5 (many diffs) n/a
4 ccsbBroadEn_13284 pDONR223 100% 49.6% 51.8% None (many diffs) n/a
5 ccsbBroad304_13284 pLX_304 0% 49.6% 51.8% V5 (many diffs) n/a
6 TRCN0000478753 CCTGCACTCTGCGGAGAACTGCCG pLX_317 100% 49.6% 51.8% V5 (many diffs) n/a
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