Construct: shRNA TRCN0000106854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005319.3-191s1c1
DNA Barcode:
GCCTCTAAAGAGCGTAGCGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H1-2 (3006)
Gene Description:
H1.2 linker histone, cluster member
Transcript:
RefSeq NM_005319.3 (CURRENT)
Match location:
Position 191 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTAAAGAGCGTAGCGGA
Hairpin Sequence:
5'-CCGG-GCCTCTAAAGAGCGTAGCGGA-CTCGAG-TCCGCTACGCTCTTTAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTAAAGAGCGTAGCGGACTCGAGTCCGCTACGCTCTTTAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTAAAGAGCGTAGCGGACTCGAGTCCGCTACGCTCTTTAGAGGC-3'

Other clones with same target sequence:

(none)