Transcript: Human NM_005319.3

Homo sapiens H1.2 linker histone, cluster member (H1-2), mRNA.

Source:
NCBI, updated 2019-07-25
Taxon:
Homo sapiens (human)
Gene:
H1-2 (3006)
Length:
732
CDS:
44..685

Additional Resources:

NCBI RefSeq record:
NM_005319.3
NBCI Gene record:
H1-2 (3006)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005319.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000424032 CAAGGTTGTCAAGCCTAAGAA pLKO_005 643 CDS 100% 5.625 7.875 N H1-2 n/a
2 TRCN0000442081 CAAGGTTGCGAAGCCCAAGAA pLKO_005 574 CDS 100% 4.950 6.930 N H1-2 n/a
3 TRCN0000437559 CGCTAAGCCCAAGGTTGTCAA pLKO_005 634 CDS 100% 4.950 6.930 N H1-2 n/a
4 TRCN0000106854 GCCTCTAAAGAGCGTAGCGGA pLKO.1 191 CDS 100% 0.220 0.308 N H1-2 n/a
5 TRCN0000443007 GCACTCTGGTGCAAACGAAAG pLKO_005 315 CDS 100% 6.000 4.200 N H1-2 n/a
6 TRCN0000106850 CCGAAGAAGAGCGCTAAGAAA pLKO.1 482 CDS 100% 5.625 3.938 N H1-2 n/a
7 TRCN0000106851 GCCGGCTATGATGTGGAGAAA pLKO.1 248 CDS 100% 4.950 3.465 N H1-2 n/a
8 TRCN0000106853 ACCAAACCTAAGAAGCCAGTT pLKO.1 419 CDS 100% 4.050 2.835 N H1-2 n/a
9 TRCN0000106852 TGTAACCAAGAAAGTGGCTAA pLKO.1 538 CDS 100% 4.050 2.835 N H1-2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005319.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00714 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00714 pLX_304 92.2% 100% 100% V5 n/a
3 TRCN0000470253 AAGGTCACGTGCCGCATGCTCGAC pLX_317 59.3% 100% 100% V5 n/a
4 ccsbBroadEn_00715 pDONR223 100% 77.4% 84.5% None (many diffs) n/a
5 TRCN0000467159 AGTATACGATAATTTCTCCAAAAA pLX_317 57.5% 77.4% 84.5% V5 (many diffs) n/a
6 ccsbBroad304_00715 pLX_304 76.1% 77.3% 84% V5 (many diffs) n/a
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