Construct: shRNA TRCN0000106929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003535.2-177s1c1
DNA Barcode:
TGAGCTGCTCATCCGCAAACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3C12 (8356)
Gene Description:
H3 clustered histone 12
Transcript:
RefSeq NM_003535.2 (CURRENT)
Match location:
Position 177 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGCTGCTCATCCGCAAACT
Hairpin Sequence:
5'-CCGG-TGAGCTGCTCATCCGCAAACT-CTCGAG-AGTTTGCGGATGAGCAGCTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGCTGCTCATCCGCAAACTCTCGAGAGTTTGCGGATGAGCAGCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGCTGCTCATCCGCAAACTCTCGAGAGTTTGCGGATGAGCAGCTCA-3'

Other clones with same target sequence:

(none)