Construct: shRNA TRCN0000108787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019754.3-667s1c1
DNA Barcode:
CCATCCTGGTTTCACAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tagln3 (56370)
Gene Description:
transgelin 3
Transcript:
RefSeq NM_019754.3 (CURRENT)
Match location:
Position 667 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCTGGTTTCACAGGAAA
Hairpin Sequence:
5'-CCGG-CCATCCTGGTTTCACAGGAAA-CTCGAG-TTTCCTGTGAAACCAGGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCTGGTTTCACAGGAAACTCGAGTTTCCTGTGAAACCAGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCTGGTTTCACAGGAAACTCGAGTTTCCTGTGAAACCAGGATGG-3'

Other clones with same target sequence:

(none)