Construct: shRNA TRCN0000109045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080470.1-3833s1c1
DNA Barcode:
CCAGTCTCTGTTTAGACCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smc1b (140557)
Gene Description:
structural maintenance of chromosomes 1B
Transcript:
RefSeq NM_080470.1 (CURRENT)
Match location:
Position 3833 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCTCTGTTTAGACCCAT
Hairpin Sequence:
5'-CCGG-CCAGTCTCTGTTTAGACCCAT-CTCGAG-ATGGGTCTAAACAGAGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCTCTGTTTAGACCCATCTCGAGATGGGTCTAAACAGAGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCTCTGTTTAGACCCATCTCGAGATGGGTCTAAACAGAGACTGG-3'

Other clones with same target sequence:

(none)