Construct: shRNA TRCN0000109183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026045.3-454s1c1
DNA Barcode:
CCAGAAGTTAACAAGGGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prpf18 (67229)
Gene Description:
pre-mRNA processing factor 18
Transcript:
RefSeq NM_026045.3 (CURRENT)
Match location:
Position 454 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGTTAACAAGGGCTTA
Hairpin Sequence:
5'-CCGG-CCAGAAGTTAACAAGGGCTTA-CTCGAG-TAAGCCCTTGTTAACTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGTTAACAAGGGCTTACTCGAGTAAGCCCTTGTTAACTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGTTAACAAGGGCTTACTCGAGTAAGCCCTTGTTAACTTCTGG-3'

Other clones with same target sequence:

(none)