Construct: shRNA TRCN0000109449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021424.2-786s1c1
DNA Barcode:
ACGCTCAACGTGCAGTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nectin1 (58235)
Gene Description:
nectin cell adhesion molecule 1
Transcript:
RefSeq NM_021424.2 (CURRENT)
Match location:
Position 786 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCTCAACGTGCAGTATGAA
Hairpin Sequence:
5'-CCGG-ACGCTCAACGTGCAGTATGAA-CTCGAG-TTCATACTGCACGTTGAGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCTCAACGTGCAGTATGAACTCGAGTTCATACTGCACGTTGAGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCTCAACGTGCAGTATGAACTCGAGTTCATACTGCACGTTGAGCGT-3'

Other clones with same target sequence:

(none)