Construct: shRNA TRCN0000110030
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_183019.2-2070s1c1
- DNA Barcode:
- GCTCTGGACATTCTTTGGAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Arhgef4 (226970)
- Gene Description:
- Rho guanine nucleotide exchange factor (GEF) 4
- Transcript:
- RefSeq NM_183019.2 (CURRENT)
- Match location:
- Position 2070 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110030)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCTGGACATTCTTTGGAAA
- Hairpin Sequence:
-
5'-CCGG-GCTCTGGACATTCTTTGGAAA-CTCGAG-TTTCCAAAGAATGTCCAGAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCTGGACATTCTTTGGAAACTCGAGTTTCCAAAGAATGTCCAGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCTGGACATTCTTTGGAAACTCGAGTTTCCAAAGAATGTCCAGAGC-3'
Other clones with same target sequence:
(none)
