Construct: shRNA TRCN0000110031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183019.2-665s1c1
DNA Barcode:
CGGGAGGATATGTTCAGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgef4 (226970)
Gene Description:
Rho guanine nucleotide exchange factor (GEF) 4
Transcript:
RefSeq NM_183019.2 (CURRENT)
Match location:
Position 665 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAGGATATGTTCAGTGAA
Hairpin Sequence:
5'-CCGG-CGGGAGGATATGTTCAGTGAA-CTCGAG-TTCACTGAACATATCCTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAGGATATGTTCAGTGAACTCGAGTTCACTGAACATATCCTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAGGATATGTTCAGTGAACTCGAGTTCACTGAACATATCCTCCCG-3'

Other clones with same target sequence:

(none)