Construct: shRNA TRCN0000110227
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_146171.1-1393s1c1
- DNA Barcode:
- GCCAGCTTTCTAGCCAATAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ncapd2 (68298)
- Gene Description:
- non-SMC condensin I complex, subunit D2
- Transcript:
- RefSeq NM_146171.1 (CURRENT)
- Match location:
- Position 1393 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110227)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCAGCTTTCTAGCCAATAAT
- Hairpin Sequence:
-
5'-CCGG-GCCAGCTTTCTAGCCAATAAT-CTCGAG-ATTATTGGCTAGAAAGCTGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCAGCTTTCTAGCCAATAATCTCGAGATTATTGGCTAGAAAGCTGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCAGCTTTCTAGCCAATAATCTCGAGATTATTGGCTAGAAAGCTGGC-3'
Other clones with same target sequence:
(none)
