Construct: shRNA TRCN0000110228

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146171.1-3116s1c1
DNA Barcode:
CCTTTGTTCCACTGCTACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncapd2 (68298)
Gene Description:
non-SMC condensin I complex, subunit D2
Transcript:
RefSeq NM_146171.1 (CURRENT)
Match location:
Position 3116 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110228)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGTTCCACTGCTACTTA
Hairpin Sequence:
5'-CCGG-CCTTTGTTCCACTGCTACTTA-CTCGAG-TAAGTAGCAGTGGAACAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGTTCCACTGCTACTTACTCGAGTAAGTAGCAGTGGAACAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGTTCCACTGCTACTTACTCGAGTAAGTAGCAGTGGAACAAAGG-3'

Other clones with same target sequence:

(none)