Construct: shRNA TRCN0000110316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173739.3-1042s1c1
DNA Barcode:
GCTGACTGAATATGTGGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt18 (233733)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 18
Transcript:
RefSeq NM_173739.3 (CURRENT)
Match location:
Position 1042 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTGAATATGTGGACAA
Hairpin Sequence:
5'-CCGG-GCTGACTGAATATGTGGACAA-CTCGAG-TTGTCCACATATTCAGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTGAATATGTGGACAACTCGAGTTGTCCACATATTCAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTGAATATGTGGACAACTCGAGTTGTCCACATATTCAGTCAGC-3'

Other clones with same target sequence:

(none)