Construct: shRNA TRCN0000110826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183319.2-169s1c1
DNA Barcode:
GCTTTGTACATGGTTAGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xkrx (331524)
Gene Description:
X-linked Kx blood group related, X-linked
Transcript:
RefSeq NM_183319.2 (CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGTACATGGTTAGAATT
Hairpin Sequence:
5'-CCGG-GCTTTGTACATGGTTAGAATT-CTCGAG-AATTCTAACCATGTACAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGTACATGGTTAGAATTCTCGAGAATTCTAACCATGTACAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGTACATGGTTAGAATTCTCGAGAATTCTAACCATGTACAAAGC-3'

Other clones with same target sequence:

(none)