Construct: shRNA TRCN0000110827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183319.2-669s1c1
DNA Barcode:
CCTGTGCAATATGTTGGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xkrx (331524)
Gene Description:
X-linked Kx blood group related, X-linked
Transcript:
RefSeq NM_183319.2 (CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGCAATATGTTGGCTAT
Hairpin Sequence:
5'-CCGG-CCTGTGCAATATGTTGGCTAT-CTCGAG-ATAGCCAACATATTGCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGCAATATGTTGGCTATCTCGAGATAGCCAACATATTGCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGCAATATGTTGGCTATCTCGAGATAGCCAACATATTGCACAGG-3'

Other clones with same target sequence:

(none)