Construct: shRNA TRCN0000111530

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030014.2-3717s1c1
DNA Barcode:
CCCAGAACATTGCCAGTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hook1 (77963)
Gene Description:
hook microtubule tethering protein 1
Transcript:
RefSeq NM_030014.2 (CURRENT)
Match location:
Position 3717 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000111530)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAACATTGCCAGTCATT
Hairpin Sequence:
5'-CCGG-CCCAGAACATTGCCAGTCATT-CTCGAG-AATGACTGGCAATGTTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAACATTGCCAGTCATTCTCGAGAATGACTGGCAATGTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAACATTGCCAGTCATTCTCGAGAATGACTGGCAATGTTCTGGG-3'

Other clones with same target sequence:

(none)