Construct: shRNA TRCN0000112422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134020.1-173s1c1
DNA Barcode:
CGAACTCAGATGTGGGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmed4 (103694)
Gene Description:
transmembrane p24 trafficking protein 4
Transcript:
RefSeq NM_134020.1 (CURRENT)
Match location:
Position 173 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAACTCAGATGTGGGACAAA
Hairpin Sequence:
5'-CCGG-CGAACTCAGATGTGGGACAAA-CTCGAG-TTTGTCCCACATCTGAGTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAACTCAGATGTGGGACAAACTCGAGTTTGTCCCACATCTGAGTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAACTCAGATGTGGGACAAACTCGAGTTTGTCCCACATCTGAGTTCG-3'

Other clones with same target sequence:

(none)