Construct: shRNA TRCN0000112460

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172712.2-4282s1c1
DNA Barcode:
CGCATTCTAAACAAGTGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Uba6 (231380)
Gene Description:
ubiquitin-like modifier activating enzyme 6
Transcript:
RefSeq NM_172712.2 (CURRENT)
Match location:
Position 4282 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112460)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATTCTAAACAAGTGCTTA
Hairpin Sequence:
5'-CCGG-CGCATTCTAAACAAGTGCTTA-CTCGAG-TAAGCACTTGTTTAGAATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATTCTAAACAAGTGCTTACTCGAGTAAGCACTTGTTTAGAATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATTCTAAACAAGTGCTTACTCGAGTAAGCACTTGTTTAGAATGCG-3'

Other clones with same target sequence:

(none)