Construct: shRNA TRCN0000113203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145368.2-267s1c1
DNA Barcode:
CCCAAGCCTTCTACAAGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acnat2 (209186)
Gene Description:
acyl-coenzyme A amino acid N-acyltransferase 2
Transcript:
RefSeq NM_145368.2 (CURRENT)
Match location:
Position 267 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGCCTTCTACAAGACTA
Hairpin Sequence:
5'-CCGG-CCCAAGCCTTCTACAAGACTA-CTCGAG-TAGTCTTGTAGAAGGCTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGCCTTCTACAAGACTACTCGAGTAGTCTTGTAGAAGGCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGCCTTCTACAAGACTACTCGAGTAGTCTTGTAGAAGGCTTGGG-3'

Other clones with same target sequence:

(none)