Construct: shRNA TRCN0000113400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013854.1-2901s1c1
DNA Barcode:
GCCTCTTGTCTCAAGACCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcf1 (224742)
Gene Description:
ATP-binding cassette, sub-family F (GCN20), member 1
Transcript:
RefSeq NM_013854.1 (CURRENT)
Match location:
Position 2901 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTGTCTCAAGACCTTT
Hairpin Sequence:
5'-CCGG-GCCTCTTGTCTCAAGACCTTT-CTCGAG-AAAGGTCTTGAGACAAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTGTCTCAAGACCTTTCTCGAGAAAGGTCTTGAGACAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTGTCTCAAGACCTTTCTCGAGAAAGGTCTTGAGACAAGAGGC-3'

Other clones with same target sequence:

  1. TRCN0000326269