Construct: shRNA TRCN0000113402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013854.1-101s1c1
DNA Barcode:
CGCGGACAAAGTAGTGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcf1 (224742)
Gene Description:
ATP-binding cassette, sub-family F (GCN20), member 1
Transcript:
RefSeq NM_013854.1 (CURRENT)
Match location:
Position 101 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGGACAAAGTAGTGAAGAA
Hairpin Sequence:
5'-CCGG-CGCGGACAAAGTAGTGAAGAA-CTCGAG-TTCTTCACTACTTTGTCCGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGGACAAAGTAGTGAAGAACTCGAGTTCTTCACTACTTTGTCCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGGACAAAGTAGTGAAGAACTCGAGTTCTTCACTACTTTGTCCGCG-3'

Other clones with same target sequence:

  1. TRCN0000326270