Construct: shRNA TRCN0000113766

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181873.1-2140s1c1
DNA Barcode:
CCCAACAAGATGGCCTAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTMR11 (10903)
Gene Description:
myotubularin related protein 11
Transcript:
RefSeq NM_181873.1 (NON-CURRENT)
Match location:
Position 2140 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113766)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACAAGATGGCCTAGAAA
Hairpin Sequence:
5'-CCGG-CCCAACAAGATGGCCTAGAAA-CTCGAG-TTTCTAGGCCATCTTGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACAAGATGGCCTAGAAACTCGAGTTTCTAGGCCATCTTGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACAAGATGGCCTAGAAACTCGAGTTTCTAGGCCATCTTGTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000299202