Construct: shRNA TRCN0000116116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002956.2-1485s1c1
DNA Barcode:
CGGGTTGAAGAAGAATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLIP1 (6249)
Gene Description:
CAP-Gly domain containing linker protein 1
Transcript:
RefSeq NM_002956.2 (CURRENT)
Match location:
Position 1485 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTTGAAGAAGAATCAATT
Hairpin Sequence:
5'-CCGG-CGGGTTGAAGAAGAATCAATT-CTCGAG-AATTGATTCTTCTTCAACCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTTGAAGAAGAATCAATTCTCGAGAATTGATTCTTCTTCAACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTTGAAGAAGAATCAATTCTCGAGAATTGATTCTTCTTCAACCCG-3'

Other clones with same target sequence:

  1. TRCN0000306993