Construct: shRNA TRCN0000120086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026152.1-1000s1c1
DNA Barcode:
GCGCTTGGATTTCAGCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoga1 (67432)
Gene Description:
4-hydroxy-2-oxoglutarate aldolase 1
Transcript:
RefSeq NM_026152.1 (CURRENT)
Match location:
Position 1000 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTTGGATTTCAGCAACAA
Hairpin Sequence:
5'-CCGG-GCGCTTGGATTTCAGCAACAA-CTCGAG-TTGTTGCTGAAATCCAAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTTGGATTTCAGCAACAACTCGAGTTGTTGCTGAAATCCAAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTTGGATTTCAGCAACAACTCGAGTTGTTGCTGAAATCCAAGCGC-3'

Other clones with same target sequence:

(none)