Construct: shRNA TRCN0000120877

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008756.2-2800s1c1
DNA Barcode:
GCTCATTTGATGTCATTAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ocln (18260)
Gene Description:
occludin
Transcript:
RefSeq NM_008756.2 (CURRENT)
Match location:
Position 2800 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000120877)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATTTGATGTCATTAGTA
Hairpin Sequence:
5'-CCGG-GCTCATTTGATGTCATTAGTA-CTCGAG-TACTAATGACATCAAATGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATTTGATGTCATTAGTACTCGAGTACTAATGACATCAAATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATTTGATGTCATTAGTACTCGAGTACTAATGACATCAAATGAGC-3'

Other clones with same target sequence:

(none)