Construct: shRNA TRCN0000121495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013375.1-1076s1c1
DNA Barcode:
GCTGGGAAGTTAATTCCTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Utp18 (217109)
Gene Description:
UTP18 small subunit processome component
Transcript:
RefSeq NM_001013375.1 (CURRENT)
Match location:
Position 1076 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGAAGTTAATTCCTGTA
Hairpin Sequence:
5'-CCGG-GCTGGGAAGTTAATTCCTGTA-CTCGAG-TACAGGAATTAACTTCCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGAAGTTAATTCCTGTACTCGAGTACAGGAATTAACTTCCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGAAGTTAATTCCTGTACTCGAGTACAGGAATTAACTTCCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000346819