Construct: shRNA TRCN0000122408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013739.1-359s1c1
DNA Barcode:
GCAAAGCTACAGCACGTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAG3L3 (442578)
Gene Description:
stromal antigen 3-like 3 (pseudogene)
Transcript:
RefSeq NM_001013739.1 (NON-CURRENT)
Match location:
Position 359 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000122408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGCTACAGCACGTCTTT
Hairpin Sequence:
5'-CCGG-GCAAAGCTACAGCACGTCTTT-CTCGAG-AAAGACGTGCTGTAGCTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGCTACAGCACGTCTTTCTCGAGAAAGACGTGCTGTAGCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGCTACAGCACGTCTTTCTCGAGAAAGACGTGCTGTAGCTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000138869
  2. TRCN0000062691