Construct: shRNA TRCN0000123521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026181.1-1192s1c1
DNA Barcode:
GCTCACGTACTTCAGGTGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpatch1 (67471)
Gene Description:
G patch domain containing 1
Transcript:
RefSeq NM_026181.1 (CURRENT)
Match location:
Position 1192 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACGTACTTCAGGTGTTA
Hairpin Sequence:
5'-CCGG-GCTCACGTACTTCAGGTGTTA-CTCGAG-TAACACCTGAAGTACGTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACGTACTTCAGGTGTTACTCGAGTAACACCTGAAGTACGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACGTACTTCAGGTGTTACTCGAGTAACACCTGAAGTACGTGAGC-3'

Other clones with same target sequence:

(none)