Construct: shRNA TRCN0000123694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133818.1-1608s1c1
DNA Barcode:
CCACTTGTATAATGCAAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
AI597479 (98404)
Gene Description:
expressed sequence AI597479
Transcript:
RefSeq NM_133818.1 (CURRENT)
Match location:
Position 1608 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTGTATAATGCAAGAAT
Hairpin Sequence:
5'-CCGG-CCACTTGTATAATGCAAGAAT-CTCGAG-ATTCTTGCATTATACAAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTGTATAATGCAAGAATCTCGAGATTCTTGCATTATACAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTGTATAATGCAAGAATCTCGAGATTCTTGCATTATACAAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000287158