Construct: shRNA TRCN0000123880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134157.2-1502s1c1
DNA Barcode:
GCGCATCTTTCCGAAAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v1b1 (110935)
Gene Description:
ATPase, H+ transporting, lysosomal V1 subunit B1
Transcript:
RefSeq NM_134157.2 (CURRENT)
Match location:
Position 1502 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCATCTTTCCGAAAGAGAT
Hairpin Sequence:
5'-CCGG-GCGCATCTTTCCGAAAGAGAT-CTCGAG-ATCTCTTTCGGAAAGATGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCATCTTTCCGAAAGAGATCTCGAGATCTCTTTCGGAAAGATGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCATCTTTCCGAAAGAGATCTCGAGATCTCTTTCGGAAAGATGCGC-3'

Other clones with same target sequence:

(none)