Construct: shRNA TRCN0000123964

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029891.2-2805s1c1
DNA Barcode:
CCCTGTGATTTCCAGGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nkrf (77286)
Gene Description:
NF-kappaB repressing factor
Transcript:
RefSeq NM_029891.2 (CURRENT)
Match location:
Position 2805 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000123964)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTGATTTCCAGGATATT
Hairpin Sequence:
5'-CCGG-CCCTGTGATTTCCAGGATATT-CTCGAG-AATATCCTGGAAATCACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTGATTTCCAGGATATTCTCGAGAATATCCTGGAAATCACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTGATTTCCAGGATATTCTCGAGAATATCCTGGAAATCACAGGG-3'

Other clones with same target sequence:

(none)