Construct: shRNA TRCN0000124223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176979.4-1722s1c1
DNA Barcode:
GCCAGAAGAGTTTCCTTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Topbp1 (235559)
Gene Description:
topoisomerase (DNA) II binding protein 1
Transcript:
RefSeq NM_176979.4 (NON-CURRENT)
Match location:
Position 1722 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAAGAGTTTCCTTGTTT
Hairpin Sequence:
5'-CCGG-GCCAGAAGAGTTTCCTTGTTT-CTCGAG-AAACAAGGAAACTCTTCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAAGAGTTTCCTTGTTTCTCGAGAAACAAGGAAACTCTTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAAGAGTTTCCTTGTTTCTCGAGAAACAAGGAAACTCTTCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000331542