Construct: shRNA TRCN0000331542
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_176979.5-1722s21c1
- DNA Barcode:
- GCCAGAAGAGTTTCCTTGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Topbp1 (235559)
- Gene Description:
- topoisomerase (DNA) II binding protein 1
- Transcript:
- RefSeq NM_176979.5 (CURRENT)
- Match location:
- Position 1722 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000331542)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCAGAAGAGTTTCCTTGTTT
- Hairpin Sequence:
-
5'-CCGG-GCCAGAAGAGTTTCCTTGTTT-CTCGAG-AAACAAGGAAACTCTTCTGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCAGAAGAGTTTCCTTGTTTCTCGAGAAACAAGGAAACTCTTCTGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCAGAAGAGTTTCCTTGTTTCTCGAGAAACAAGGAAACTCTTCTGGC-3'
Other clones with same target sequence:
-
TRCN0000124223
