Construct: shRNA TRCN0000124370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134139.1-299s1c1
DNA Barcode:
CCCTTATCACATGTGTGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr74 (107071)
Gene Description:
WD repeat domain 74
Transcript:
RefSeq NM_134139.1 (CURRENT)
Match location:
Position 299 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTATCACATGTGTGGATT
Hairpin Sequence:
5'-CCGG-CCCTTATCACATGTGTGGATT-CTCGAG-AATCCACACATGTGATAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTATCACATGTGTGGATTCTCGAGAATCCACACATGTGATAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTATCACATGTGTGGATTCTCGAGAATCCACACATGTGATAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000288096