Construct: shRNA TRCN0000124528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030742.1-1497s1c1
DNA Barcode:
CCACCAGAGAATGAACCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r63 (81017)
Gene Description:
vomeronasal 1 receptor 63
Transcript:
RefSeq NM_030742.1 (CURRENT)
Match location:
Position 1497 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCAGAGAATGAACCACAT
Hairpin Sequence:
5'-CCGG-CCACCAGAGAATGAACCACAT-CTCGAG-ATGTGGTTCATTCTCTGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCAGAGAATGAACCACATCTCGAGATGTGGTTCATTCTCTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCAGAGAATGAACCACATCTCGAGATGTGGTTCATTCTCTGGTGG-3'

Other clones with same target sequence:

(none)