Construct: shRNA TRCN0000124742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028762.1-722s1c1
DNA Barcode:
GCTGTCAGACATGGACTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm19 (74111)
Gene Description:
RNA binding motif protein 19
Transcript:
RefSeq NM_028762.1 (CURRENT)
Match location:
Position 722 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000124742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCAGACATGGACTACTT
Hairpin Sequence:
5'-CCGG-GCTGTCAGACATGGACTACTT-CTCGAG-AAGTAGTCCATGTCTGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCAGACATGGACTACTTCTCGAGAAGTAGTCCATGTCTGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCAGACATGGACTACTTCTCGAGAAGTAGTCCATGTCTGACAGC-3'

Other clones with same target sequence:

(none)