Construct: shRNA TRCN0000125066

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198634.1-528s1c1
DNA Barcode:
CGCTGGAAACGTCGAAACAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tigd3 (332359)
Gene Description:
tigger transposable element derived 3
Transcript:
RefSeq NM_198634.1 (CURRENT)
Match location:
Position 528 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125066)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGGAAACGTCGAAACAAT
Hairpin Sequence:
5'-CCGG-CGCTGGAAACGTCGAAACAAT-CTCGAG-ATTGTTTCGACGTTTCCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGGAAACGTCGAAACAATCTCGAGATTGTTTCGACGTTTCCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGGAAACGTCGAAACAATCTCGAGATTGTTTCGACGTTTCCAGCG-3'

Other clones with same target sequence:

(none)