Construct: shRNA TRCN0000125732

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175386.3-536s1c1
DNA Barcode:
GCCTGTATCCTTTGGCACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lhfp (108927)
Gene Description:
lipoma HMGIC fusion partner
Transcript:
RefSeq NM_175386.3 (CURRENT)
Match location:
Position 536 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000125732)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTATCCTTTGGCACTTT
Hairpin Sequence:
5'-CCGG-GCCTGTATCCTTTGGCACTTT-CTCGAG-AAAGTGCCAAAGGATACAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTATCCTTTGGCACTTTCTCGAGAAAGTGCCAAAGGATACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTATCCTTTGGCACTTTCTCGAGAAAGTGCCAAAGGATACAGGC-3'

Other clones with same target sequence:

(none)