Construct: shRNA TRCN0000126410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001011732.1-1726s1c1
DNA Barcode:
GCCTGTCATTCGGATTGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xkr7 (228787)
Gene Description:
X-linked Kx blood group related 7
Transcript:
RefSeq NM_001011732.1 (CURRENT)
Match location:
Position 1726 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000126410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTCATTCGGATTGACTT
Hairpin Sequence:
5'-CCGG-GCCTGTCATTCGGATTGACTT-CTCGAG-AAGTCAATCCGAATGACAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTCATTCGGATTGACTTCTCGAGAAGTCAATCCGAATGACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTCATTCGGATTGACTTCTCGAGAAGTCAATCCGAATGACAGGC-3'

Other clones with same target sequence:

(none)