Construct: shRNA TRCN0000127306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026382.2-228s1c1
DNA Barcode:
GCATGCCCAAATCATCACTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snrnp48 (67797)
Gene Description:
small nuclear ribonucleoprotein 48 (U11/U12)
Transcript:
RefSeq NM_026382.2 (CURRENT)
Match location:
Position 228 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGCCCAAATCATCACTAA
Hairpin Sequence:
5'-CCGG-GCATGCCCAAATCATCACTAA-CTCGAG-TTAGTGATGATTTGGGCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGCCCAAATCATCACTAACTCGAGTTAGTGATGATTTGGGCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGCCCAAATCATCACTAACTCGAGTTAGTGATGATTTGGGCATGC-3'

Other clones with same target sequence:

(none)