Construct: shRNA TRCN0000127308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026382.2-185s1c1
DNA Barcode:
GCCGAGGATGAAATCGCCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snrnp48 (67797)
Gene Description:
small nuclear ribonucleoprotein 48 (U11/U12)
Transcript:
RefSeq NM_026382.2 (CURRENT)
Match location:
Position 185 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAGGATGAAATCGCCATA
Hairpin Sequence:
5'-CCGG-GCCGAGGATGAAATCGCCATA-CTCGAG-TATGGCGATTTCATCCTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAGGATGAAATCGCCATACTCGAGTATGGCGATTTCATCCTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAGGATGAAATCGCCATACTCGAGTATGGCGATTTCATCCTCGGC-3'

Other clones with same target sequence:

(none)