Construct: shRNA TRCN0000127694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022756.3-746s1c1
DNA Barcode:
GCCTGTGACTTTGAGTAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEAF6 (64769)
Gene Description:
MYST/Esa1 associated factor 6
Transcript:
RefSeq NM_022756.3 (NON-CURRENT)
Match location:
Position 746 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000127694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTGACTTTGAGTAGTTT
Hairpin Sequence:
5'-CCGG-GCCTGTGACTTTGAGTAGTTT-CTCGAG-AAACTACTCAAAGTCACAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTGACTTTGAGTAGTTTCTCGAGAAACTACTCAAAGTCACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTGACTTTGAGTAGTTTCTCGAGAAACTACTCAAAGTCACAGGC-3'

Other clones with same target sequence:

(none)