Construct: shRNA TRCN0000129559

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_060969.2-1893s1c1
DNA Barcode:
CGAGACAGCTACAAGTCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C10orf71 (118461)
Gene Description:
chromosome 10 open reading frame 71
Transcript:
RefSeq XM_060969.2 (NON-CURRENT)
Match location:
Position 1893 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129559)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGACAGCTACAAGTCCAAA
Hairpin Sequence:
5'-CCGG-CGAGACAGCTACAAGTCCAAA-CTCGAG-TTTGGACTTGTAGCTGTCTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGACAGCTACAAGTCCAAACTCGAGTTTGGACTTGTAGCTGTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGACAGCTACAAGTCCAAACTCGAGTTTGGACTTGTAGCTGTCTCG-3'

Other clones with same target sequence:

(none)