Construct: shRNA TRCN0000131011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176811.2-3346s1c1
DNA Barcode:
CCACAGAACCTCAGCTTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NLRP8 (126205)
Gene Description:
NLR family pyrin domain containing 8
Transcript:
RefSeq NM_176811.2 (CURRENT)
Match location:
Position 3346 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGAACCTCAGCTTTGAA
Hairpin Sequence:
5'-CCGG-CCACAGAACCTCAGCTTTGAA-CTCGAG-TTCAAAGCTGAGGTTCTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGAACCTCAGCTTTGAACTCGAGTTCAAAGCTGAGGTTCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGAACCTCAGCTTTGAACTCGAGTTCAAAGCTGAGGTTCTGTGG-3'

Other clones with same target sequence:

(none)