Construct: shRNA TRCN0000131075

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023012.4-1540s1c1
DNA Barcode:
GCATGGGTGTTGCATTGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSRC2 (65117)
Gene Description:
arginine and serine rich coiled-coil 2
Transcript:
RefSeq NM_023012.4 (NON-CURRENT)
Match location:
Position 1540 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131075)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGGTGTTGCATTGACTT
Hairpin Sequence:
5'-CCGG-GCATGGGTGTTGCATTGACTT-CTCGAG-AAGTCAATGCAACACCCATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGGTGTTGCATTGACTTCTCGAGAAGTCAATGCAACACCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGGTGTTGCATTGACTTCTCGAGAAGTCAATGCAACACCCATGC-3'

Other clones with same target sequence:

(none)