Construct: shRNA TRCN0000134892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015264.1-619s1c1
DNA Barcode:
CCCAACATCTTCTTCATGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIAA0930 (23313)
Gene Description:
KIAA0930
Transcript:
RefSeq NM_015264.1 (CURRENT)
Match location:
Position 619 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACATCTTCTTCATGATT
Hairpin Sequence:
5'-CCGG-CCCAACATCTTCTTCATGATT-CTCGAG-AATCATGAAGAAGATGTTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACATCTTCTTCATGATTCTCGAGAATCATGAAGAAGATGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACATCTTCTTCATGATTCTCGAGAATCATGAAGAAGATGTTGGG-3'

Other clones with same target sequence:

(none)