Construct: shRNA TRCN0000135317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152755.1-336s1c1
DNA Barcode:
CTTACAGCGTTTCAGAGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNPY4 (245812)
Gene Description:
canopy FGF signaling regulator 4
Transcript:
RefSeq NM_152755.1 (NON-CURRENT)
Match location:
Position 336 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTACAGCGTTTCAGAGACAA
Hairpin Sequence:
5'-CCGG-CTTACAGCGTTTCAGAGACAA-CTCGAG-TTGTCTCTGAAACGCTGTAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTACAGCGTTTCAGAGACAACTCGAGTTGTCTCTGAAACGCTGTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTACAGCGTTTCAGAGACAACTCGAGTTGTCTCTGAAACGCTGTAAG-3'

Other clones with same target sequence:

(none)