Construct: shRNA TRCN0000140572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017528.2-626s1c1
DNA Barcode:
GACTACCCTAACAGTGCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BUD23 (114049)
Gene Description:
BUD23 rRNA methyltransferase and ribosome maturation factor
Transcript:
RefSeq NM_017528.2 (NON-CURRENT)
Match location:
Position 626 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000140572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTACCCTAACAGTGCCAAA
Hairpin Sequence:
5'-CCGG-GACTACCCTAACAGTGCCAAA-CTCGAG-TTTGGCACTGTTAGGGTAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTACCCTAACAGTGCCAAACTCGAGTTTGGCACTGTTAGGGTAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTACCCTAACAGTGCCAAACTCGAGTTTGGCACTGTTAGGGTAGTC-3'

Other clones with same target sequence:

  1. TRCN0000275217